烟草甲对S-烯虫酯的抗药性风险评估

Resistance assessment of Lasioderma serricorne to S-methoprene

中文关键词:  烟草甲  S-烯虫酯  抗药性风险评估  现实遗传力

英文关键词:Lasioderma serricorne  S-methoprene  resistance risk assessment  realized heritability

中文摘要:

      为评估烟草甲对S-烯虫酯的抗药性风险,本研究采用室内群体汰选法筛选其抗性种群,测定不同浓度S-烯虫酯处理下F0至F13代烟草甲的化蛹率与羽化率变化,并运用Tabashnik域性状分析法计算其抗性现实遗传力(h2),进而预测烟草甲对S-烯虫酯的抗性发展速率。结果表明,在F7至F13代,不同浓度S-烯虫酯处理下,幼虫化蛹率均无显著差异,且均高于90%。在特定浓度处理下,F1至F13代烟草甲的羽化率随汰选世代增加呈上升趋势。经13代室内选育,烟草甲对S-烯虫酯的LC50由0.12 mg/kg上升至0.91 mg/kg,抗性倍数达7.3倍,平均抗性现实遗传力为0.1328,表明存在一定的抗性风险。本研究结果为S-烯虫酯的合理使用提供了理论依据。

英文摘要:

      To assess the resistance risk ofSLasioderma serricorneSto S-methoprene, this study employed an indoor population selection method to establish a resistant population. The pupation and emergence rates of the F0 to F13 generations ofSL. serricorneSwere determined under different concentrations of S-methoprene. The realized heritability (h2) of resistance was calculated using Tabashnik’s threshold trait analysis method to predict the resistance development rate ofSL. serricorneSto S-methoprene. The results showed that from the F7 to F13 generations, there were no significant differences in larval pupation rates among the different S-methoprene concentration treatments, and all pupation rates exceeded 90%. Under specific concentration treatments, the emergence rate of the F1 to F13 generations ofSL. serricorneSshowed an increasing trend with successive selection generations. After 13 generations of indoor selection, the LC₅₀ ofSL. serricorneSto S-methoprene increased from 0.12 mg/kg to 0.91 mg/kg, representing a resistance ratio of 7.3-fold, with a mean realized heritability of 0.1328, indicating a certain level of resistance risk. These findings provide a theoretical basis for the rational use of S-methoprene.