基于斑马鱼活体生物传感器模型的山核桃油生理功效研究

Physiological efficacy of wild pecan oil based on a zebrafish in vivo biosensor model

中文关键词:  山核桃油  斑马鱼  生物传感器模型  润肠通便  抗细胞衰老  多模态生理功效

英文关键词:Wild pecan (Carya cathayensis)  Zebrafish  Biosensor model  Laxative effect  Anti-aging  Innate immunity  Multimodal physiological efficacy

中文摘要:

      本研究利用斑马鱼活体生物传感器模型，系统性评价了野生山核桃油在润肠通便、抗细胞衰老及增强先天免疫方面的多模态生理功效。建立了三种斑马鱼模型：（1）硫酸铝诱导便秘模型（5 dpf），以钙黄绿素染色评估肠道荧光强度变化；（2）活性氧（ROS）模型（3 dpf），通过DCFH-DA染色检测ROS水平；（3）转基因中性粒细胞荧光品系模型（3 dpf），定量中性粒细胞数量及荧光强度。研究发现山核桃油处理组的肠道荧光强度较模型组显著降低46.2%（P?< 0.05），ROS荧光强度较空白组降低38.7%（P?< 0.05），接近谷胱甘肽组水平，且中性粒细胞数量较空白组增加52.4%（P?< 0.05），且显著高于对照组。试验结果表明野生山核桃油通过促进肠道蠕动、清除活性氧及激活中性粒细胞，同步实现润肠通便、抗衰老和增强先天免疫的三重功效，为开发功能性食用油提供了实验依据。

英文摘要:

      This study systematically evaluated the multimodal physiological efficacy of wild pecan oil in promoting laxative effects, combating cellular senescence, and enhancing innate immunity using a zebrafish in vivo biosensor model. Three zebrafish models were established: (1) An aluminum sulfate-induced constipation model (5 dpf), assessing intestinal motility via calcein staining intensity; (2) A reactive oxygen species (ROS) model (3 dpf), detecting ROS levels using DCFH-DA staining; (3) A transgenic neutrophil fluorescent line [Tg(lyz:DsRed)] (3 dpf), quantifying neutrophil count and fluorescence intensity. Results demonstrated that the pecan oil-treated group exhibited a 46.2% reduction (P < 0.05) in intestinal fluorescence intensity compared to the model group. ROS fluorescence intensity decreased by 38.7% (P < 0.05) versus the blank group, approaching glutathione group levels. Neutrophil count increased by 52.4% (P < 0.05) compared to the blank group and significantly exceeded the control group. These findings indicate that wild pecan oil simultaneously achieves tripartite efficacy—laxative effects, anti-aging, and enhanced innate immunity—through promoting intestinal peristalsis, scavenging ROS, and activating neutrophils, providing experimental evidence for developing functional edible oils.